No heating-time programming – you must visually judge melting. This is the PC-10’s biggest limitation (and simplicity).
If you have a PC‑100 or PC‑100C, please specify — I can write a separate guide for those digital models.
Narishige PC-10 is a dual-stage vertical micropipette puller designed for high-reproducibility fabrication of microinjection needles and patch-clamp electrodes. While the Narishige PC-100
is the updated compatible replacement, the PC-10 remains a laboratory staple due to its robust manual controls. NARISHIGE GROUP Core Functionality & Operation Dual-Stage Pulling:
Automates the transition from a first "thinning" pull to a second final pull for consistent tip geometry. Stabilized Power Supply:
Features a built-in digital display of output voltage to minimize variation and improve reproducibility. Gravity-Based Pull:
Uses specific weight blocks (light and heavy) to vary the pulling force. Acrylic Shroud:
Includes a transparent cover to shield the glass capillaries from air currents during the heating process. NARISHIGE WEB NEWS Typical Settings for Common Pipettes Settings vary by glass type, but general rules apply: NARISHIGE WEB NEWS High Heater Value: Produces long, thin pipettes. Low Heater Value: Produces short, thick pipettes. Double Pull:
Typically yields "firm" pipettes suitable for patch-clamping or penetrative injections. NARISHIGE WEB NEWS Troubleshooting & Error Messages If your unit is displaying an error code, refer to the official Narishige FAQ for guidance:
Heater unit moving distance is set to 0mm; set to at least 1mm. First pull not detected; increase No. 1 heater output.
Slider descending too fast; reduce No. 1 heater output or adjust weights.
Poor contact or broken heater; tighten fixing screws or replace the filament. NARISHIGE GROUP Maintenance Tips
The Narishige PC-10 is a high-precision vertical needle puller designed for the production of glass micropipettes
. It operates using gravitational force and a heating element to pull glass capillaries into fine tips for applications such as patch clamping and microinjection. 1. Key Features & Specifications Pull Modes : Supports both Single Pull (one continuous motion) and Double Pull (automated two-stage process for complex shapes). Digital Display
: Shows digital values for heater and electromagnet levels (0–100 scale) to ensure high repeatability. Precision Control
: Includes two types of weights (light and heavy) for fine tension adjustment. Technical Data Heater Level : 100 = 2.5V heater voltage. Power Source : 100V–240V AC (±5%). Dimensions : W205 x D190 x H185mm; Weight: Approx. 6.0kg. 2. Safety & Handling Precautions
: Install on a level, horizontal surface in a non-humid environment. Maintenance
: Clean blood or chemical stains with organic solvents like alcohol; do not boil for sterilization. Disassembly
: Do not loosen internal structure clamps or attempt to disassemble for repair. 3. Operational Procedures Initial Setup
: Ensure the instrument is perfectly horizontal to maintain pull symmetry. Heater Block Adjustment
: Set the heater block according to the glass capillary diameter.
: Place the glass capillary into the clamps securely without over-tightening. Double Pull Mode First Pull
: Adjust the position plate and set the "No.1 Heater" level. The capillary will pull down to a thin, tapered state. Automated Reset
: The heater unit will move to a second position automatically. Second Pull
: Set the "No.2 Heater" level. This stage finalizes the tip diameter and shape. 4. Error Messages & Troubleshooting Common error codes for the PC-10 include: : Slider distance set to 0mm. : Set to 1mm or longer. : First pull completion not detected. : Increase No.1 Heater output. : Slider speed too fast during first pull. : Reduce No.1 Heater output or weight. : Heater contact failure or broken heater. : Tighten fixing screws or replace heater. narishige pc10 manual new
For further detailed setup values for specific pipette shapes, refer to the Narishige Customer Support News No.051 official PC-10 user manual PDF calculating specific heater settings based on your glass capillary dimensions? No.051 Setting Values in the PC-10 Puller (Revised)
A standout feature for the Narishige PC-10 Go to product viewer dialog for this item.
(often referred to as a manual or vertical puller) is its automated dual-stage pulling mode, which significantly improves the reproducibility of specialized pipette tips.
While simple in design, this system allows you to preset two different heating levels—one for the initial stretch and another for the final pull—enabling the production of everything from long, thin microinjection needles to firm, blunt microelectrodes for patch-clamping. Key Specifications & Features
Gravity-Fed Tensioning: It uses a classic vertical "free fall" method with two pairs of interchangeable weights (two light and two heavy) to provide consistent pulling force without the complexity of electromagnetic coils.
Stabilized Power Supply: The unit includes a built-in power source designed to minimize voltage variations, ensuring the heater element delivers uniform current for every pull.
Digital LED Display: A clear readout shows the exact heater level status, making it easy to mass-produce pipettes with identical tip geometries once you've found your ideal settings.
Protective Acrylic Shroud: An integrated cover shields the glass capillary from air currents and drafts during the pulling process, preventing external environmental factors from affecting the final tip shape.
Fine Control: The heater control uses a ten-turn adjusting knob for ultra-precise settings, allowing for tip sizes ranging from several tens of micrometers down to sub-micron levels.
Detailed operational settings and maintenance tips can be found in the Narishige PC-10 User Manual.
The workshop smelled of solvent and warm plastic. Under a single swinging lamp, Lena unfolded a brittle manual labeled NARISHIGE PC-10—its corners softened by oil and years—then, with the care of someone handling a relic, set it beside the device.
The PC-10 looked deceptively simple: a compact pipette controller, its molded grip worn where fingers had learned its comforting contours. Yet Lena knew it held a kind of quiet authority in the lab—a small instrument that, in skilled hands, could direct tiny lives and measure impossibly small worlds. The manual’s title—“NARISHIGE PC-10—NEW”—was printed in a font that somehow promised both reassurance and challenge.
She’d inherited the controller and the manual from Dr. Sato, who’d guided her through her first awkward hours at the bench. He’d called it “the storyteller of precision,” and Lena had never forgotten that phrase. Tonight, alone, she wanted to learn it herself—every button, every whisper of function—and perhaps to discover why the lab’s coffee machine broke exactly the day Sato left.
Page by page, the manual revealed a domestic map: diagrams for battery insertion, diagrams for operating modes—aspirate, dispense, fine-adjust—each icon small and patient. The “NEW” edition included a note about calibration: a reminder that even instruments must be taught to be honest. Lena smiled. She’d calibrated people before; calibrating a device felt like the same kind of conversation.
She clicked the power and felt the controller vibrate with hidden readiness. In the dim glow, the LED panel blinked a soft green. The manual described an optional replacement tip; Lena fitted one and pretended it was a pen. She had a recipe to make: a line of diluted dye to trace through a microchannel, so thin it would show how the controller breathed.
The first draw was hesitant. She watched the dye climb inside the pipette tip like a tide. The manual’s step-by-step instructions read like a ritual, each sentence insisting on patience. “Hold steady,” it said—no, Lena imagined it said—“hold steady, and let the instrument learn your rhythm.” She regulated the flow with the thumb dial until the bead of liquid floated, then released. Precision was a quiet miracle.
Her phone buzzed. A message from New Lab Supplies: the PC-10 was discontinued. Lena’s fingers pressed the controller more tightly. The manual’s language—technician-forward, exacting—felt suddenly more intimate, a testament against obsolescence. It had been a tool for a generation of careful hands, each note in the margins a fingerprint: a tiny smear at “sterilize before use,” a pencil tick by “troubleshooting—air leak,” a dotted line connecting a page number to a memory.
Lena turned to troubleshooting and found what she needed: common faults, clear cures, diagrams of the inner valves. She had been told that Dr. Sato had once dismantled the PC-10 during a midnight storm and reassembled it with a superstition of care. She imagined him, flashlight in teeth, reading the very pages she now held.
She ran a test series—a slow aspirate, a fast dispense, a micro-adjust to find the sweet spot between too slow and too violent. The manual’s charts suggested optimal speeds; Lena found them conservative, affectionate. She skimmed “maintenance” and vowed to clean the seals tonight. The manual ended with a quiet paragraph about responsibility: instruments carried memory, and the lab was a place where those memories mattered.
When she finally set the manual down, dawn touched the rim of the window. The PC-10 sat between her hands like a small orbiting planet—simple, steadfast, full of potential. The “NEW” on the cover no longer felt like a marketing claim but a promise pinned to a moment: tools renewed by knowledge, by the willingness of one more hand to learn.
Outside, the city stirred. Inside, Lena closed the manual and placed it back in its box, writing in the margin of the title page: “Calibrated 04-09-2026. —L.” It was a notation small as a comma, but in time it would become a lineage, a tiny lineage recorded in pencil on paper, passed down like an instruction and a blessing.
Later, when a new student asked where to find the PC-10 manual, Lena would hand them the box and say, simply: “Read it. Then teach it to your hands.”
Review: Narishige PC-10 Micropipette Puller (New Unit)
Rating: ★★★★½ (4.5/5)
The Bottom Line: The Narishige PC-10 is the "Toyota Hilux" of micropipette pullers—it isn't flashy, it lacks a digital touchscreen, and it looks like it was designed in 1995, but it is incredibly reliable, easy to fix, and produces consistent patches day in and day out. If you are tired of fighting with the programming menus of the Sutter P-97, this analog workhorse is a breath of fresh air.
Ease of Use & Setup: The biggest selling point for new users is the simplicity. Out of the box, setup took about 20 minutes. There is no complex software interface. You are greeted with two simple heater settings (Heater 1 and Heater 2) and a weight adjustment dial. For a lab transitioning from a broken older model, the learning curve is virtually non-existent. The "new" manual included is concise and actually helpful—unlike some translated technical manuals that are incomprehensible. The schematics for filament alignment were clear and accurate.
Performance: We use this primarily for standard patch-clamp electrodes (borosilicate glass, 1.5mm OD).
Build Quality: This is built like a tank. The mechanical solenoid is loud (a distinct CLACK sound), but it feels industrial and durable. The filament access is easy to open, making cleaning and filament replacement painless. The unit takes up a small footprint on the bench, which is a bonus for crowded electrophysiology rigs.
Pros:
Cons:
Final Verdict: If you need a "set it and forget it" puller for routine patch-clamping, the Narishige PC-10 is an excellent investment. It strips away the unnecessary digital complexity of modern pullers and focuses on the physics of melting glass. Highly recommended for labs that value reliability over bells and whistles.
Note: If by "PC10" you actually meant the PC-10 hydraulic micromanipulator or a specific accessory part, please clarify, but the above review applies to their most popular item bearing that model designation.
Narishige PC-10 Go to product viewer dialog for this item. is a dual-stage, vertical glass micropipette puller designed for high-precision laboratory applications such as patch clamping and microinjection. While it has been largely superseded by the digital
, the PC-10 remains a robust tool for creating tips ranging from sub-micron sizes to tens of microns. Core Functionality and Modes
The PC-10 utilizes gravity-fed vertical pulling to ensure uniformity. It features a mode-selector knob that allows users to toggle between four primary operations: STEP 1 (Single-Stage):
Pulls the glass capillary in one continuous stretch. This mode is typically used to create longer, more supple pipettes with larger tip diameters. STEP 2 (Two-Stage):
Automates a mid-process change in settings. The first pull thins the glass, and the second pull finishes the tip, resulting in firm, parallel-shaped electrodes ideal for patch clamp experiments. HEATER 1 & 2:
These settings allow the user to display and adjust the specific heating values for each pulling stage on the digital LED readout. Operational Parameters
The final shape of the pipette is determined by three main variables:
The Narishige PC-10 is a dual-stage, vertical-pull micropipette puller designed for high-precision laboratory tasks such as patch-clamping and microinjection. Mastering the latest operational protocols ensures consistent pipette geometry and extends the life of your heating filament. Key Features and Specifications
The PC-10 utilizes gravity-based pulling with adjustable weights to create varied pipette shapes.
Dual Pulling Modes: Features automated one-stage (single stretch) and two-stage (mid-process setting change) modes for versatility.
Digital Accuracy: Digital displays show heater output (0–100), where 100 represents approximately 2.5V, enabling repeatable mass production.
Precision Weighting: Includes two light (approx. 25g) and two heavy (approx. 100g) weights for fine-tuning tension.
Safety Features: Includes an acrylic cover and a dedicated power switch that illuminates the heater level display. Operating Instructions
According to the Narishige PC-10 Operation Manual, effective operation follows these core steps:
Preparation: Ensure the heater coil is centered around the glass capillary. Misalignment can lead to off-center or distorted tips.
Mode Selection: Use the Mode-selector knob to choose between STEP 1 (one-stage) or STEP 2 (two-stage). No heating-time programming – you must visually judge
Heater Adjustment: Use the NO. 1 and NO. 2 heater adjusting knobs to set specific heat levels for each stage.
Execution: Press the START button to begin the automated pull. Protocol Tips for Common Needs Desired Pipette Type Suggested Settings (Reference Only) Long, Thin Injection Needles High heating value + One-stage pulling Firm Patch-Clamp Electrodes Low heating value + Two-stage pulling Flexible, Sharp Tips Use all available weights to increase pull speed Gradual, Parallel Taper Use fewer weights (e.g., 2 Type Light) Maintenance and Troubleshooting NARISHIGE WEB NEWS Setting Values in the PC-10 Puller - NARISHIGE WEB NEWS
Here’s an interesting, story-driven write-up based on the search query "narishige pc10 manual new" — tailored for a blog, forum post, or product listing.
The PC-10 doesn’t need a software update. It needs a guide that speaks to the modern tinkerer. The rise of "narishige pc10 manual new" as a search term isn’t about buying a fresh machine—it’s about resurrecting a classic with fresh documentation.
So if you find one of those new community manuals? Treasure it. Print it. Laminate it. And maybe one day, pass it on to the next scientist who rescues a dusty PC-10 from the shelf.
Paper: Optimized Calibration and Operation of the Narishige PC-10 Vertical Puller 1. Introduction
The Narishige PC-10 is a high-precision vertical puller designed to produce long, thin microneedles or firm microelectrodes. It operates on a vertical axis, using the gravitational force of internal weights to pull heated glass. 2. Technical Specifications & Modes
Pulling Mechanism: Uses a combination of heater output and interchangeable weights (light and heavy). Operating Modes:
Single Pull: Pulls the capillary in one continuous stretch for specific needle shapes.
Double Pull: Changes heater or tension settings mid-process automatically, ideal for patch pipettes.
Materials: Compatible with borosilicate glass capillaries, such as the Narishige GD-1 with internal filaments. 3. Calibration & Setup (Manual Guidelines)
Proper setup is critical for reproducibility. Users often reference technical guides from NARISHIGE WEB NEWS to determine starting values.
Heater Values: The display shows digital values (0–100) representing output power.
Weight Adjustment: Changing the number of weights (e.g., using 2 vs. 4 weights) drastically alters the pipette’s angle and parallelism.
Environmental Compensation: Pulling temperatures must be adjusted based on ambient lab temperature and the "age" of the heating filament. 4. Operational Best Practices
Expert users on platforms like ResearchGate suggest specific workflows:
Filament Maintenance: Replace filaments every 1–2 years or when heating becomes inconsistent.
Contact Cleaning: If heater function is irregular, polish the contact surfaces between the heater block and mounting plates with sandpaper.
Two-Step Refinement: Use a high first-stage temperature to initiate melting and a lower second-stage temperature to refine the tip resistance. 5. Maintenance and Troubleshooting
Error Handling: If the slider falls prematurely in two-stage mode, check the First Pull Terminating Position Adjusting Slider and ensure the solenoid coil is functioning.
Filament Care: Ensure heater coils do not touch; use a wrench to separate them or replace the unit if the heater is deteriorated. 6. Conclusion
The PC-10 remains a reliable standard for microelectrode fabrication. Its versatility is maximized through precise weight adjustment and the automated double-pull mode, provided the user performs regular Heater Block maintenance.
| Issue | Cause | Solution (per manual) | |-------|-------|----------------------| | Glass drops without pulling | Weight too heavy for heat | Increase heat or reduce weight | | Bulbous tip | Heat too high / too slow | Increase weight, lower heat slightly | | Asymmetric tips | Filament not centered or glass tilted | Realign filament, clean chucks | | Filament burns out fast | Current too high | Use lower heat, replace every 200 pulls | | No separation | Glass ID too large | Use thinner wall glass (<0.6 mm ID) |
Durability: Known to last 20+ years with occasional filament changes. The new manual emphasizes cleaning the rails monthly. If you have a PC‑100 or PC‑100C ,