Biosu42 «SECURE – 2024»
Before you begin, you need to know exactly what motherboard you have. Do not guess, as installing the wrong BIOS can "brick" your computer.
Assume the executable is biosu42 and input files are FASTA or FASTQ.
Convert formats (FASTA ↔ FASTQ or filtering by quality): biosu42
biosu42 convert --input reads.fastq --output reads.fasta
biosu42 convert --input reads.fastq --output highq.fastq --min-mean-q 30
Filter sequences by length or pattern:
biosu42 filter --input sequences.fasta --min-len 200 --max-len 2000 --output filtered.fa
biosu42 filter --input sequences.fasta --motif ATGCGT --output motif_hits.fa
Batch processing with BED intervals:
biosu42 extract --input genome.fa --bed intervals.bed --output extracted_regions.fa
Compute k-mer frequencies:
biosu42 kmers --input sequences.fasta --k 5 --top 20
Assuming biosu42 is distributed via pip: Before you begin, you need to know exactly
If it’s provided as a binary release, download the appropriate archive, unpack, make executable, and add to PATH:
tar -xzf biosu42-<version>-linux.tar.gz
chmod +x biosu42
mv biosu42 /usr/local/bin/